Journal: Immunologic research

Article Title: FGL2 knockdown improves heart function through regulation of TLR9 signaling in the experimental autoimmune myocarditis rats

doi: 10.1007/s12026-017-8965-4

Figure Lengend Snippet: Western blotting analysis for TLR9 and Fgl2 protein expression in rat myocardial tissue. a TLR9 and Fgl2 protein expression stripe in each group. TLR9 protein expression in myocardial tissue of the EAM group was significantly higher than that of the control group on day 21 after initial immunization. TLR9 protein expression was significantly lower in the RNAi group compared with the EAM group, and also significantly higher in the RNAi group compared with the NC group. Fgl2 protein expression in myocardial tissue of the EAM group was significantly higher than that in the NC group on day 21 after initial immunization. Fgl2 protein expression was significantly lower compared with those in EAM and GFP groups. b β-Actin as control; the TLR9/β-actin and Fgl2/β-actin ratio of NC was considered as 100%. *P < 0.05 vs NC, group, #P < 0.05 vs EAM group

Article Snippet: Antibodies and reagents Recombinant virus’ plasmid and the carriers of auxiliary packaging original plasmids were purchased from Shanghai Jikai Gene Chemical Co.. PCR re-agents were as follows: TRIzol kits were purchased from Invitrogen, retrovirus RNA kits from Promega, and RNase free from Axygen; PCR primer sequences were designed and synthesized by Shanghai Promega Biological company; P1250 Protein Extraction Kit I was purchased from Applygen Technologies Inc.; rat anti-Fgl2 antibody was purchased from Sigma; rat anti-TLR9 antibody was purchased from Novus Biologicals; cardiac myosin of pigs’ heart was purchased from Sigma, complete Freund’s adjuvant CFA was purchased from Sigma; and rat anti-β-actin antibody and goat anti-rat IgG antibody were purchased from ZSGB-BIO.

Techniques: Western Blot, Expressing, Control